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Home > 학회활동 > 국제학술지 |
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작성일 : 13-05-13 18:54
| 저 자 |
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Do-Wan Lee et al |
| 제 목 |
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Quantitative assessment of neurochemical changes in a rat model of long-term alcohol consumption as detected by in vivo and ex vivo proton nuclear magnetic resonance spectroscopy |
| 학회지명 |
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Neurochemistry International |
| 권 호 |
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62 |
| 페이지 |
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502-509 |
| 년,월 |
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2013-5-13 |
| 링 크 |
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 http://www.ncbi.nlm.nih.gov/pubmed/23411411 [1578] |
| Abstract |
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The aim of present study was to quantitatively investigate the neurochemical profile of the frontal cortex
region in a rat model of long-term alcohol consumption, by using in vivo proton magnetic resonance spectroscopy
(1H-MRS) at 4.7 T and ex vivo 1H high-resolution magic angle spinning (HR-MAS) technique at
11.7 T. Twenty male rats were divided into two groups and fed a liquid diet for 10 weeks. After 10 weeks,
in vivo 1H MRS spectra were acquired from the frontal cortex brain region. After in vivo 1H MRS experiments,
all animals were sacrificed and 20 frontal cortex tissue samples were harvested. All tissue examinations
were performed with the 11.7 T HR-MAS spectrometer and high-resolution spectra were
acquired. The in vivo and ex vivo spectra were quantified as absolute metabolite concentrations and normalized
ratios of total signal-intensity (i.e., metabolitesNorm), respectively. The absolute quantifications of
in vivo spectra showed significantly higher glycerophosphocholine plus phosphocholine (GPC + PCh) and
lower myo-inositol (mIns) concentrations in ethanol-treated rats compared to controls. The quantifications
of ex vivo spectra showed significantly higher PChNorm, ChoNorm and tChoNorm, and lower GPCNorm
and mInsNorm ratio levels in ethanol-treated rats compared to controls. Our findings suggest that reduced
mIns concentrations caused by the long-term alcohol consumption may lead to hypo-osmolarity syndrome
and astrocyte hyponatremia. In addition, increased choline-containing compound concentrations
may reflect an increased cell turnover rate of phosphatidylcholine and other phospholipids, indicating an
adaptive mechanism. Therefore, these results might be utilized as key markers in chronic alcohol intoxication
metabolism. |
 2013_이도완_NI.pdf (672.5K) [1577] DATE : 2013-05-13 18:57:50 |
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